Study on genetic diversity of natural and cultivated Cistanche tubulosa / 中国中药杂志

<p><b>OBJECTIVE</b>To determine the genetic diversity of natural and cultivated Cistanche tubulosa.</p><p><b>METHOD</b>123 individuals of six populations of C. tubulosa, including four natural populations and two cultivated ones, were analyzed by random amplified polymorphic DNA (RAPD) markers to determine the genetic variations among the populations.</p><p><b>RESULT</b>A total of 87 loci (including 24 polymorphic loci) were amplified using 10 random primers. The average percentage of polymorphic loci (PPL) was 27.59 in the natural populations. The PPL between natural populations are 19.54 to 25.29. Of the four natural populations, Andi' er population had highest PPL (25.29). The two cultivated populations had low PPL (13.79 and 11.49). Cluster analysis using UPGMA revealed that populations of natural and cultivated were separated into two groups, the four natural populations clustered as one group and the two cultivated populations clustered as another group, indicating that the natural and cultivated populations had obvious differentiation.</p><p><b>CONCLUSION</b>In view of the low genetic diversity of the cultivated C. tubulosa, we strongly suggested that the natural populations should be conserved in particular.</p>

The determination of echinacoside and acteoside in herbs of Cistanche tubulosa / 中国中药杂志

<p><b>OBJECTIVE</b>To analyse the echinacoside and acteoside content of Cistanche tubulosa, collected from different hosts and different size of the cultivated, which is compared to the wilding by RP-HPLC method.</p><p><b>METHOD</b>An Agilent Eclipse XDB-C18(4.6 mm x 250 mm, 5 microm) column was used and a mixture of methanol-acetonitrile-1% acetic acid (15:10:75) was used as the mobile phase at a flow rate of 0.6 mL x min(-1). The column temperature was 30 degrees C and the UV detection wavelength was 334 nm.</p><p><b>RESULT</b>The calibration curves of echinacoside and acteoside were in good linearity over the range of 0.904-9.04 microg (r = 0.999 9), and 1.27-12.7 microg (r = 0.999 9) respectively and the average recoveries of echinacoside (and acteoside) were 98.9% (n = 5, RSD 1.9%), and 97.0% (n = 5, RSD 0.97%).</p><p><b>CONCLUSION</b>The method is simple, quick, acurate. In all of the samples, the contents of echinacoside is markedly more than that of acteoside, the content of the two active component in the wilding is higher than that in the planting. The content of sample in the different sizes gradually increase from the big to the small, and the contents of samples collected from the different hosts vary markedly. These results are useful for the quality evaluation of medicinal materials of C. tubulosa.</p>